The invention relates to a robot automatic inspection and analysis system and a using method. The robot automatic inspection and analysis system comprises a box body, a touch screen, a display screen, a monitor, a titrating device, a test tube feeding device, a test tube heating device, an exhausting device and a control device; the box body consists of an upper box and a lower box; the upper box is of a transparent and sealed structure; the titrating device and the monitor are arranged at the top of the upper box; a manipulator, the test tube feeding device and the test tube heating device are fixed to the interior of the upper box; the test tube feeding device and the test tube heating device are annularly arranged on the periphery of the manipulator; the exhausting device is arranged at the lower box; the touch screen and the display screen are fixed to the box body; the control device is arranged in the lower box; the monitor directly transmits image information to a specified Internet through built-in wifi. The robot automatic inspection and analysis system and the using method have the advantages that a robot serves as an inspection and analysis operator, so that in most work of inspection and analysis, the robot replaces manpower to operate, and automatic inspection and analysis are realized with the cooperation of an intelligent control system.