The invention discloses a probabilistic load flow calculation method considering static frequency characteristic uncertainty. The method comprises the steps of establishing a probabilistic load flow calculation model considering static frequency characteristic uncertainty of a generator set and a load by combining an idea of a dynamic load flow according to a characteristic of primary frequency modulation performance uncertainty of load generation in a power system; performing analytical calculation by combining Monte Carlo sampling and a cumulant method as well as Gram-Charlier expansion series; and providing probability distribution of active power output of the load and a generator and probability distribution of active power load flows of lines after a fault of the system. Therefore, a calculated result is accurate and close to a running status of an actual power grid.