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发明授权
CN106801052B 一种猪等孢球虫rRNA基因序列及其获取方法 有权

一种猪等孢球虫rRNA基因序列及其获取方法
摘要：
本发明涉及生物基因工程技术领域，根据Genbank上Is 18S rRNA基因全序列(U97523.1)及Is ITS1基因序列(LC085519.1)设计引物，以Is基因组DNA(gDNA)为模板，PCR方法扩增获得Is‑18S rRNA基因序列；根据所获得Is‑18S rRNA及Is‑ITS1基因序列，分别设计三条同向且退火温度较高的特异性引物，利用Genome walking技术克隆获得Is‑18S‑ITS1‑5.8S‑ITS2‑28S rRNA基因全长序列，从而为猪等孢球虫新一代检测方法的建立打下基础。
摘要（英）：

The invention relates to the technical field of biology gene engineering. According to Is 18S rRNA gene complete sequence (U97523.1) and Is ITS1 gene sequence (LC085519.1) design primers on a Genbank, the Is-18S rRNA gene sequence can be obtained by using an Is gDNA (genome deoxyribonucleic acid) as a template through PCR (polymerase chain reaction) method amplification; three homodromous specific primers with high annealing temperature are respectively designed according to the obtained Is-18S rRNA gene sequence and the Is ITS1 gene sequence; a Genome walking technology is used for cloning to obtain the Is-18S-ITS1-5.8S-ITS2-28S rRNA gene complete sequence, so that the foundation is laid for a isospora suis new generation detecting method.

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