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发明公开
CN106801109A 一种猪非典型瘟病毒RT‑PCR检测特异性引物、试剂盒及检测方法 有权

一种猪非典型瘟病毒RT‑PCR检测特异性引物、试剂盒及检测方法
摘要：
本发明属于动物病毒学及分子生物学技术领域，具体涉及一种猪非典型瘟病毒RT‑PCR检测特异性引物、试剂盒及检测方法。所述特异性引物核苷酸序列如SEQ ID NO:1和SEQ ID NO:2所示；所述试剂盒包括所述引物、Prime Script 1 step酶混合物、2×1 step缓冲液和无核酸酶水。本发明还公开了利用所述特异性引物或所述试剂盒检测猪非典型瘟病毒的方法。本发明引物特异性和灵敏度高，可以于各种临床样本中检测、鉴别出猪非典型瘟病毒，从而可以快捷及时地采取防治措施，减少经济损失。
摘要（英）：

The invention belongs to the technical fields of animal virology and molecular biology, and particularly relates to a pig atypical pestivirus RT-PCR detection specific primer, a kit and a detection method. The nucleotide sequences of the specific primer are as shown in SEQ ID NO:1 and SEQ ID NO:2; the kit comprises the primer, a Prime Script 1 step enzyme mixture, a 2*1 step buffer solution and nuclease-free water. The invention also discloses a method for detecting pig atypical pestivirus by using the specific primer or the kit. The primer provided by the invention has high specificity and sensitivity and can be used for detecting and identifying the pig atypical pestivirus from various clinical samples, so that prevention and control measures can be taken quickly and timely, and economic loss is reduced.

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