The invention discloses a kit for promoting in-vitro proliferation of radix campanumoeae. The kit comprises a culture medium 1 used for inducing a stem section with adventives buds and a culture medium 2 used for repeated subculture, and each of the culture medium 1 and the culture medium 2 comprises 6-benzylaminopurine, thidiazuron and MS culture liquid. In addition, the invention discloses a method for promoting in-vitro proliferation of radix campanumoeae. By adopting the kit and the method, the problems that proliferated seedlings are thin, weak, withered and yellow and dead and proliferation rate is low when proportioning of NAA and BA is adopted for proliferation are solved; 30 days is taken as a culture cycle, proliferation rate of radix campanumoeae can reach 10-15 times by utilizing the kit, robust aseptic seedlings can be obtained, and either rooting rate or transplanting survival rate are 100%.