The invention discloses an anti-stress method of temporarily culturing coilia ectenes in small body, which is characterized by including the following steps: (1) the temporarily culturing tool is that an open vessel is adopted; (2) the elimination of noise is that an elastic sound insulation material is added to the position where the open vessel touches the ground; (3) the water regulation is that aquaculture water for coilia ectenes is added into the open vessel, wherein the aquaculture water for coilia ectenes is ensured that the dissolved oxygen is higher than 7.0mg/L, the pH is 7.0 to 7.6, the temperature of the water is lower than 25 DEG C, the mass ratio of sorbitol is 0.6% to 1.2%, and the mixed water is dissolved sufficiently by stirring; (4) coilia estenes are transported to the open vessel for temporarily culturing with water and a shading device should be installed at the mouth of the open vessel, thereby reducing light and stress. The using tools of the anti- stress method of temporarily culturing coilia ectenes in small body are simple, which are conventional instruments in aquaculture and convenient to obtain. The anti-stress agent used is a single component and innocuous and harmless. The cost of the anti-stress agent is low, but the effect of anti-stress is remarkable. The anti-stress agent can raise the survival ratio of six hours of the temporarily culturing small bodies to more than 96%.