The invention provides an acidification method of a defueling pump well and a defueling pump well tubular column, and aims to realize well bottom acidification plugging removal via the defueling pump well tubular column. The defueling pump well tubular column comprises a first tubular column and a second tubular column arranged in a sleeve pipe, wherein the first tubular column and the second tubular column are arranged at intervals from top to bottom; the first tubular column is connected with a tubing hanger of a wellhead; the second pipe column is fixed to the sleeve pipe. The acidification method comprises the following steps: pumping acid liquor to an oil sleeve annular space between the first tubular column and the sleeve pipe, so that the acid liquor flows in the second pipe column via the oil sleeve annular space, and enters a barefoot interval stratum under the second tubular column via the second tubular column; pumping clean water to the oil sleeve annular space between the oil pipe and the sleeve pipe, and extruding the acid liquor in the oil sleeve annular space in the barefoot interval stratum. According to the acidification method of the defueling pump well and the defueling pump well tubular column disclosed by the embodiment of the invention, the acidification plugging removal is realized; during the process of oil production, repeatedly lifting and sinking of the tubular column by a well service hoist are not needed; the construction working procedures are simplified, and the operating cost is reduced.