The invention discloses a nutrient constant-basis setup apparatus for a stem cell culture medium. The nutrient constant-basis setup apparatus comprises a sealed container, a partition, an air guiding hose and sponge. The partition divides the sealed container into a left chamber and a right chamber, and the left chamber and the right chamber are communicated through the bottom of a partition wall; an extractor hole is formed in the top of the left chamber, and a rubber plug is arranged in the extractor hole; an inlet hole is arranged in the top of the right chamber, and an air filtration sterilization unit is arranged in the inlet hole; a liquid outlet is arranged in the bottom of the right chamber and is connected to the stem cell culture medium through a guide tube; the sponge floats in the left chamber; the air guiding hose penetrates the partition with one end located in the right chamber and the other end arranged at the bottom of the sponge; the total length of the air guiding hose located in the left chamber is greater than the height of the left chamber. By the design, minimal fluctuation of the liquid surface can be guaranteed.