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发明公开
CN106834543A 人疱疹病毒各亚型快速检测与定量试剂和试剂盒 有权

人疱疹病毒各亚型快速检测与定量试剂和试剂盒
摘要：
本发明公开一种人疱疹病毒各亚型快速检测试剂，包括特异性扩增HSV‑1 RL2，HSV‑2 UL28，VZV ORF26，EBV EBNA1，HCMV IE，HHV‑6A或HHV‑6B U22，HHV‑6A或HHV‑6B IE，HHV‑7 U95和KSHV ORF72的正反向引物和相应内参标品；还公开了一种包括上述检测试剂的人疱疹病毒各亚型快速检测试剂盒；还公开了一种包括上述检测试剂的人疱疹病毒各亚型快速定量试剂以及检测与定量试剂盒。本发明可针对不用来源的感染早期临床样本进行快速、有效的各亚型鉴别与诊断，还可方便、准确地进行病毒拷贝数定量计算，具有很高的临床诊断使用价值。
摘要（英）：

The invention discloses a reagent for rapidly detecting various subtypes of human herpes viruses. The reagent comprises forward and reverse primers specifically amplifying HSV-1 RL2, HSV-2 UL28, VZV ORF26, EBV EBNA1, HCMV IE, HHV-6A or HHV-6B U22, HHV-6A or HHV-6B IE, HHV-7 U95 and KSHV ORF72 and a corresponding internal reference standard. The invention also discloses a kit which contains the detection reagent and is used for rapidly detecting various subtypes of the human herpes viruses and also discloses a reagent which contains the detection reagent and is used for rapidly quantifying the various subtypes of the human herpes viruses as well as a detection quantification kit. The reagent disclosed by the invention can rapidly and effectively identify and diagnose the various subtypes on clinical samples in early stage of infection and with different sources, also can conveniently and accurately carry out virus copy number quantitative calculation and has high clinical diagnosis use value.

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