A dosimetric smoke determination method used in a full-smoke exposure experiment is used for real-time and quantitative determination of smoke dosage in a full-smoke exposure bin through combination of a quartz micro balance wafer and an inserted cell culture dish. The method has the maximum characteristic that an actual microenvironment for smoke exposure is simulated, the actual concentration (including the mass concentration of smoke particulate matter and nicotine concentration) of cigarette smoke in the full-smoke exposure bin is detected in a real-time, sensitive and quantitative mode, the dosage-effect relation of a smoke toxicity effect is accurately represented, and a real smoke dosage basis is provided for evaluation of cigarette smoke in-vitro toxicology. By applying method, the toxicity mechanism of the cigarette smoke can be better interpreted, and a method basis and technical support are provided for health risk assessment and research of tobacco products. In addition, the method is wide in applicability and can be applied to in-vitro toxicity testing and research of cigarettes, electronic cigarettes, cigarettes non-combustible in heating and aerosols different in source, and testing results are good in repeatability, high in stability and strong in sensitivity.