The invention provides a method and device for adjusting the flicker of a display panel, and a display device and belongs to the technical field of display. The method can solve a problem that a conventional method for adjusting the image flicker of a liquid crystal display panel cannot fundamentally improve the image display quality. The method for adjusting the flicker of a display panel comprises: inverting the original polarity of data voltage input into at least a part of the data lines of the display panel when it is detected that the display panel displays a flickering image so as to adjust the image flicker; scanning an I2C interface and determining whether or not the writing of common voltage exists; and if yes, restoring the polarity of the data voltage on the data line to the original polarity to adjust the flickering image again.