The invention relates to the field of insecticides, in particular to a preparation for preventing and controlling bemisia tabaci, and a preparation and application method of the preparation. A technical scheme adopted for solving the technical problems is as follows: the preparation for preventing and controlling the bemisia tabaci is characterized by comprising active ingredients of 40 parts of butyl hydroxybenzoate and 60 parts of matrine. The preparation and application method of the preparation for preventing and controlling the bemisia tabaci comprises the following steps: mixing the butyl hydroxybenzoate and the matrine according to a ratio; preparing any dosage form applicable to the agriculture by utilizing a known method, wherein a better dosage form is a suspending agent, suspoemulsion, micro emulsion, emulsion in water, aqueous dispersion granules, micro-capsule suspending agent or dispersible oil suspending agent. The prepared preparation is added into a sprayer, and then the preparation is sprayed by the sprayer; and a backpack-type motion manual sprayer is adopted as follows: the volume is 15L, 6 to 8 times are pressed per minute, and the flow rate is 0.2 to 0.4 L/min.