The invention discloses a manufacturing method of touch screen cover plates. According to the manufacturing method, an integral body protection film is prepared on the surface of a substrate; the integral body protection film is subjected to exposure developing so as to form a button protection film matching a part for setting of a button and the shape of the button; the substrate is subjected to roughening treatment so as to form surface microstructures on the substrate not covered by the button protection film; the substrate is subjected to smoothing treatment so as to reduce the thickness of the substrate; a 3D button is formed under the button protection film; and the button protection film is removed. The manufacturing method is capable of simplifying manufacturing steps, wear resistance is improved, and user experience is improved.