The invention discloses a long wavelength type thiophenol fluorescent probe as well as a preparation method and application thereof. According to the long wavelength type thiophenol fluorescent probe, amino porphyrin and 2,4-dinitrobenzenesulfonyl chloride are used as raw materials and proper reaction conditions are controlled to obtain a target probe compound. The fluorescent probe disclosed by the invention has a very long fluorescence emission wavelength (lambda-em is more than 650nm), rapid response and high anti-interference capability; high-selectivity and high-sensitivity fluorescent recognition and detection on trace thiophenol in a water environment and a biological sample can be realized; the long wavelength type thiophenol fluorescent probe has very good application prospect.