The invention discloses primers and a TaqMan probe for triplet fluorescent quantitative PCR detection of aquatic animal derived bacterium sulfonamide-resistance genes; the primers and the probe include three groups of primers and one probe in accordance with the aquatic animal derived bacterium sulfonamide-resistance genes Sul1, Sul2 and Sul3, wherein base sequences of the three groups of primers and probes are shown as SEQ ID No.1-SEQ ID No.9 in a sequence list. Therefore, the inventor, by optimizing reaction conditions through taking a recombinant plasmid standard as a template, establishes a triplet fluorescent quantitative PCR method, which is capable of simultaneously detecting the three drug-resistance genes and tests the method from sensitivity, specificity, repeatability and clinical strain detection; results show that the method has the characteristics of being rapid, simple and convenient, and accurate; and the method is applicable to clinical detection of sulfonamide-resistance genes of bacteria; therefore, a completely new technical means is provided for rapid detection of sulfonamide resistance and for investigation of the drug-resistance genes.