The invention relates to an experimental platform for water and soil loss determination. The experimental platform comprises two slide rails which are arranged on the ground in parallel; a separating plate is movably blocked in a sliding slot of each slide rail; the front ends of the two slide rails are vertically resisted against a side wall of a water draining channel respectively; a storage tank is arranged in the middle of the side wall of the water draining channel between the two side rails; a filter screen is horizontally arranged on the middle part of the storage tank; a water draining hole which is connected with the water draining channel is formed in the side wall of the storage tank under the filter screen; a connecting plate is arranged at the top of the side wall of the storage tank on the other side of the water draining hole; the connecting plate is moveably connected with the side wall of the storage tank through a rotating shaft; and a tray is arranged on the upper side of the connecting plate which is connected with the storage tank. The experimental platform is reasonable in structure design; the meadows at different gradients are used for performing rainfall, air blowing and dust flying experiments; the information collection is accurate; the survival rate of grass seeds is increased; the experimental platform has significance in further protecting and planning the natural meadows; the working efficiency is greatly increased; and the problems of the prior art are solved.