The invention discloses a microsecond pulse plasma jet all-in-one machine, which comprises a microsecond pulse excitation power supply, a plasma jet device and a reaction gas device, and is characterized in that the microsecond pulse excitation power supply is connected with a high-voltage end of the plasma jet device through a high-voltage wire arranged in a connecting wire, and a reaction gas in the reaction gas device gets into a gas inlet of the microsecond pulse excitation power supply through a gas inlet pipe and gets into the plasma jet device through a gas outlet pipe arranged in the connecting wire. The invention further provides an application method of the microsecond pulse plasma jet all-in-one machine. The beneficial effects are that the microsecond pulse plasma jet all-in-one machine is of an integrated design, has the advantages of high discharge efficiency and device miniaturization, and is convenient to carry and use; the microsecond pulse plasma jet all-in-one machine can output high-voltage pulses with the voltage amplitude being continuously adjustable within 0-20kV, the frequency being continuously adjustable within 0-1.5kHz, the rising edge being 0.5[mu]s and the full width at half maximum being 8[mu]s; and the microsecond pulse plasma jet all-in-one machine can e applied to the fields of surface treatment, biomedical applications and the like in practical application.