The invention discloses a preparation method of E0-level melamine modified urea formaldehyde resin. The E0-level melamine modified urea formaldehyde resin is prepared by the following steps of feeding urea by three batches, and feeding melamine by three batches. The invention also discloses the E0-level melamine modified urea formaldehyde resin and an E0-level melamine modified urea formaldehyde resin adhesive. The method has the advantages that the distribution of the internal molecular structure of the urea formaldehyde resin is changed by the multi-batch feeding type, the prepared resin is added into an adhesive mixer, a formaldehyde removing agent, a reinforcing agent, an acid curing agent and industrial flour are stirred until all the components are uniformly mixed, so as to obtain a mixed adhesive, and the obtained mixed adhesive is applied into the production of parquets; the hot pressing cycle can be shortened, the hot pressing time is shortened from conventional 70-79s/mm to 30-35s/mm under the condition of no change of pressure and temperature parameters, and the utilization rate and production efficiency of a hot press are greatly improved; the release amount of formaldehyde in the prepared plate reaches superE0 level.