The present invention relates to a lateral ventricle puncture operation rehearsal device. The device comprises a skull model, the skull model is composed of a cranium model located at the upper portion of a position between eyebrows and a skull main body located at the lower portion of a position between the eyebrows, the cranium model and the skull main body can be detachably connected, the cranium model is internally provided with simulation brain tissue, the simulation brain tissue is internally provided with a simulation lateral ventricle, the simulation lateral ventricle is internally provided with a pressure sensor, a water inlet rubber pipe and a water outlet rubber pipe, an operation area module is arranged on the cranium model, and the cranium model at the operation area module comprises from outside to inside in order: a simulation skin layer, a simulation bone bed and a simulation endocranium layer. The structure is simple.