The invention discloses a transition tank type transfer window with a spraying disinfection device. A window body of the transition tank type transfer window consists of an upper box, two mechanically-interlocked pressing type airtight doors, a high-pressure magnetic pump, an atomizing spray head, a soaking tank box, two mechanically-interlocked pressing type sealing covers, a separating plate, an electromagnetic valve, a water drain valve and the like; a transferred object is transferred from a low-level cleaning area to a high-level cleaning area, and is disinfected by an ultraviolet lamp, adisinfection liquid atomizing mode and the like, or the surface of the object is soaked with disinfection liquid by the transition tank to be disinfected; the disinfection medicine liquid is contained into the transition tank, and liquid level detection, liquid level display and low liquid level alarming functions are provided; an object transfer device is arranged in the transition tank. The transition tank type transfer window has the advantage that by adopting a professional sealing system and a built-in disinfection system, a traditional transfer window and a transition tank can be effectively integrated, so that the laboratory space is saved, the convenience in use is realized, and the safety and reliability are realized.