The invention discloses an anti-fluffing finishing agent for embroidery and a use method thereof. The anti-fluffing finishing agent comprises a copolymer, fibroin powder, a surface active agent and deionized water, wherein the copolymer consists of a methacrylamide monomer, a methacrylate monomer, a butyl acrylate monomer and an acrylic-2-hydroxyethyl methacrylate monomer. The use method comprises the following steps of: selecting a writing brush with a proper size to dip a proper amount of anti-fluffing finishing agent for embroidery, uniformly brushing along embroidery patterns multiple times, each in a small amount, till the embroidery patterns are fully wet so as to prevent the finishing agent from permeating to substrates outside the embroidery patterns, then drying the embroidery product by hot air with the temperature of 50-80 DEG C, finally baking the dried embroidery product for 3-5 minutes by using hot air with the temperature of 120-150 DEG C, and after treatment is finished, ironing by using an iron. The anti-fluffing finishing agent and the use method thereof disclosed by the invention have the advantages that after anti-fluffing finishing is carried out on an embroidery product, the fluffing performance of the embroidery product can be greatly improved, and the durability and the quality of the embroidery product can be improved.