The present invention relates to a guano simulation liquid preparation device. The device comprises a fixed support (6), a preparation container is hanged and installed on the fixed support (6), a piezoelectric transducer (3) is installed on the preparation container, the piezoelectric transducer (3) is connected with an ultrasonic power supply (7) to drive the preparation container to shock, a raw material addition port (1) and a wash port (2) are arranged at the top of the preparation container, an outlet (5) is arranged at the bottom of the preparation container, and a first valve is installed at the raw material addition port (1), a second valve is installed at the wash port (2), and a third valve is installed at the outlet (5); and the preparation container is internally provided with a conductivity tester (4), and the output end of the conductivity tester (4) is connected with a display or a computer (8) arranged at the external portion of the preparation container through a connection line. The guano simulation liquid preparation device can drive the shock of the preparation container so as to prompt the rapid and uniform mixing of raw materials.