The invention relates to a pixel structure and a manufacturing method thereof as well as a display panel, aiming at solving the problem of how to improve the resolution ratio of the display panel. The pixel structure comprises a plurality of triangular sub-pixels; one pixel unit is formed by four sub-pixels with different colors; the colors of any two sub-pixels, which commonly utilize the same triangular edge, are different; each row and each column of the sub-pixels are composed of upright triangular sub-pixels and inverted triangular sub-pixels through alternated arrangement; six columns of adjacent sub-pixels form one group; each row comprises four sub-pixels with different colors and arrangement manners of each group are the same; the upright triangular sub-pixels are other sub-pixels with three different colors; the inverted triangular sub-pixels are sub-pixels with a first color. According to the pixel structure, the arrangement manner of each sub-pixel is tighter; the quantity of the sub-pixels can be increased under the condition that the size of the display panel is not changed and the physical resolution ratio of the display panel is improved.