The invention belongs to the technical field of researches of Ti-based materials and particularly relates to a three-dimensional graduated TiOF2 microsphere constructed from multiple levels of nanosheets and a preparation method and an application of the three-dimensional graduated TiOF2 microsphere. The three-dimensional graduated TiOF2 microsphere constructed from multiple levels of nanosheets is in a three-dimensional graduated microsphere structure comprising an inner core, a middle layer and an outer layer, wherein the diameter of the inner core is 1-1.5 mum, the inner core comprises a TiOF2 nanoparticle with the diameter of 5-20 nm, the middle layer is wrapped outside the inner core and comprises the TiOF2 nanosheets radially arranged outwards along the inner core, the outer layer comprises a layered TiOF2 nanosheet wrapped on the outer surface of the middle layer, the morphology is unique, the degradation effect of methylene blue is excellent under natural light, the raw materials are easy to get in the preparation, the cost is relatively low, the reaction conditions are mild, the process is simple, the scope of application of the TiOF2 is expanded, and the problem of high difficulty in degradation of methylene blue is solved.