The invention provides an efficient detection method of the activity of tobacco pollens. By taking a solution containing 10 percent of saccharose, 150 mg/L of boric acid and 20 mg/L of calcium chloride, or containing 3 percent of glucose, 50 mg/L of boric acid and 20 mg/L of calcium chloride as a culture solution, dark box moisturizing culture is performed for 3 hours, and the size of the activity of the tobacco pollen can be quantitatively judged according to the situation that pollens are germinated to generate an effective pollen tube. According to the efficient detection method, the activity of the fresh tobacco pollens can be detected and authenticated to be 86.8 and 91.3 percent, which is very close to the activity level of fresh pollens. The method is the most efficient and most reliable detection method for authenticating the activity of the tobacco pollens at present.