The invention discloses unbleached bamboo pulp non-woven and a preparation method thereof. The natural, unbleached, harmless and environment-friendly bamboo pulp is mixed with other textile staples as a raw material, and the bamboo pulp is adhered or bonded to the other textile staples to build three-dimensional net woven braided structure non-woven again. The non-woven has an enough width and length, consistent knitting compactness of various parts and consistent thickness as textile fabrics, strength indexes of a woven fabric are achieved, meanwhile, destroy to a natural bamboo fabric is reduced, protection of the length and the morphological structure of the natural bamboo fabric is facilitated, ''bamboo kun (Chinese character)'' is prevented from being destroyed to the maximal extent and the unbleached bamboo pulp non-woven has natural antibacterial, anti-mite and odor-resistant functions.