The invention discloses a Pizhou white garlic tissue culture method and a culture medium composition and relates to the technical field of garlic tissue culture. The method comprises carrying out bulb tip induction culture through Pizhou white garlic bulb tip as an explant, carrying out leaf induction culture on the leaves to obtain callus tissue, and carrying out callus differentiation culture and rooting culture on the callus tissue to obtain a complete Pizhou white garlic tissue culture seedling. The tissue culture method can effectively improve the survival rate of a bulb tip and reduce a pollution rate during operation, has a high proliferation coefficient of 42.5 and is conducive to the industrialized production of Pizhou white garlic seeds and seedlings.