利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN106939300A 树鼩肝细胞永生化细胞系及其构建方法与应用 无效 - 撤回

树鼩肝细胞永生化细胞系及其构建方法与应用
摘要：
本发明涉及一种树鼩肝细胞永生化细胞系及其构建方法与应用，属于生物医学技术领域。该细胞系命名为ITH6.1，保藏于中国典型培养物保藏中心建株，保藏号为CCTCC NO:C201740，保藏地址为湖北省武汉市武昌区八一路299号武汉大学。本发明的ITH6.1永生化树鼩肝细胞系可连续传代100次后，仍然保持二倍体核型，具备极强的增殖能力，真正实现了永生化。该细胞有着较强的增殖能力，进一步对ITH6.1进行蛋白表达、生化指标等特性分析，结果显示了ITH6.1具有典型的树鼩肝细胞特性。同时致瘤性实验等安全性检测也提示了该细胞系具有较好的生物安全性。
摘要（英）：

The invention relates to a tree shrew hepatocyte immortalized cell line and its construction method and application and belongs to the technical field of biomedicine. The cell line is named as ITH6.1, is preserved in the China center for type culture collection in the Wuhan University at No. 229, Bayi road, Wuchang District, Wuhan City, Hubei Province and has a preservation number CCTCC NO: C201740. After continuous passage 100 times, the tree shrew hepatocyte immortalized cell line ITH6.1 still keeps a diploid karyotype and has a very strong multiplication capacity so that immortalization is realized really. The cell line has a strong multiplication capacity and realizes protein expression and biochemical index characteristic analysis of ITH6.1. The result shows that the ITH6.1 has typical tree shrew hepatocyte characteristics. The safety test such as a tumorigenicity test also indicates that the cell line has better biosecurity.

0/0