The invention belongs to the technical field of marine environmental monitoring, and discloses a marine pollution early warning method based on marine biological behavior response, and the method is as follows: selecting marine environment parameters, determining biological indicators, setting an early warning threshold value; establishing an early warning model; collecting marine environment parameter values and biological behavior response data; receiving the parameter values and the data by a computer; comparing the parameter values and the set threshold value; if the parameter value exceeds the threshold value range, feeding back and outputting ''pollution'' by the computer, and determining the degree of the pollution; if the computer feeds back and outputs ''normal seawater environment'', comparing the data and the set early warning threshold value, if the data is in the threshold value range, feeding back and outputting '' normal biological indicators'' by the computer, if the data is not in the threshold value range, feeding back and outputting ''pollution'' by the computer, and determining the degree of the pollution. A three-grade early-warning structure of two types of exceeding-standard marine environmental parameter and abnormal biological behavior response is established in the method, and the method has the advantages of clear arrangement, high efficiency, comprehensiveness, high accuracy and good operability.