The invention discloses bait for strengthening cultivation of fry and a preparation method thereof and relates to the field of fishery breeding. The bait comprises, by mass, 60 to 80% of fermented soybean meal, 5 to 15% of yeast cell wall, 1 to 8% of live lactobacillus, 1 to 8% of photosynthetic bacteria and 5 to 15% of chlorella powder. After entering water, the bait can fast form floccule and promote the rapid propagation of rotifers in the water and provide a natural food for the fry. The live lactobacillus and photosynthetic bacteria can adjust the growth of effective microbial community in the intestinal tract of the fry, can eliminate anti-nutrients, can improve activity of protease, amylase and lipase in fry, can significantly improve the intestinal digestibility of fry, can prevent fry diseases and death caused by intestinal discomfort and can promote the growth rate and survival rate of fry.