The invention discloses a portable test tube stand. The portable test tube stand comprises a test tube stand body, wherein a framework is arranged at the outer side of the test tube stand body; an upper fixed plate is arranged at the inner side of the upper end of the framework; a middle fixed plate is arranged at the inner side of the middle of the framework; a bottom plate is arranged at the bottom of the framework; cover plate brackets are arranged between two placement holes on the upper fixed plate; cover plates are arranged at two sides of the upper ends of the cover plate brackets; buttons with the quantity which is the same as that of bases are arranged at one side of the bottom plate; sleeves are arranged at the lower sides of the bases; a buckle is arranged at one side of each sleeve; a connecting plate is arranged at the lower end of each sleeve; resetting springs are arranged at two ends below each connecting plate; fixed plates are arranged at the bottoms of the resetting springs; inner sleeves are arranged at the upper sides of the fixed plates; cover plates are arranged at the upper side of the test tube stand and has an effect of shielding a test tube opening, so that the volatilization of a reagent is stopped and impurities are prevented from entering test tubes; an ejection device and a button combination are arranged at the bottom of the test tube stand and ejection is controlled through the buttons, so that the test tubes are rapidly and conveniently taken out and are not easily taken by mistake.