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发明公开
CN106947801A 一种东风螺致病性弧菌的快速显色培养基及利用该培养基的检测方法 审中-实审

一种东风螺致病性弧菌的快速显色培养基及利用该培养基的检测方法
摘要：
本发明公开了一种东风螺致病性弧菌的快速显色培养基，每1000mL东风螺致病性弧菌的快速显色培养基中含有蛋白胨10～15g、牛肉膏3～5g、蔗糖10～15g、氯化钠15～25g、青霉素0.05～0.07g、链霉素1～2mg、克林霉素1～2mg、四环素0.025～0.05mg、利福平0.05～0.08mg、苯酚红0.025～0.05g，最终pH为8.0～8.2。该显色培养基能针对东风螺致病性弧菌进行快速判别和分离培养。本发明还提供了一种利用该东风螺致病性弧菌的快速显色培养基进行东风螺致病性弧菌的检测和估数的方法，该方法特异性强、灵敏度高、耗时短、成本低。
摘要（英）：

The invention discloses a quick chromogenic medium for pathogenic vibrios of babylonia lutosa. 1000 ml of the quick chromogenic medium includes: 10-15 g of peptone, 3-5 g of beef extract, 10-15 g of sucrose, 15-25 g of NaCl, 0.05-0.07 g of penicillin, 1-2 mg of streptomycin, 1-2 mg of clindamycin, 0.025-0.05 mg of tetracycline, 0.05-0.08 mg of rifampicin and 0.025-0.05 g of phenol red, final pH being 8.0-8.2. The quick chromogenic medium, aiming to the pathogenic vibrios of babylonia lutosa, can perform quick determination and separation and cultivation. The invention also provides a method of detection and number estimation of the pathogenic vibrios of babylonia lutosa by means of the quick chromogenic medium. The method has strong specificity and high sensitivity, is short in time consumption and is low in cost.

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