The invention aims at researching and developing a fluorescent sensor quick detection microorganism for starlike cyclodextrin, and screening an antibiotics antibacterial mechanism of a microorganism film. A complex is combined with a statistics analysis tool to specifically identify a pathogenic microorganism film; the complex is combined with a feature microorganism film, the surface fluorescent protein on the surface are free under the competition action of the feature microorganism, and a feature response signal can be obtained according to different bonding strength and mutual action force of each microorganism film; on the basis of experiment, the complex is further applied to the evaluation and screening of the antibiotic mechanism. The fluorescent sensor quick detection microorganism for starlike cyclodextrin has the advantages that the starlike quaternary ammonium salt cyclodextrin and the fluorescent protein can form the complex to detect the microorganism film, any pretreatment of the microorganism film system is not needed, only simple steps are required, and the reference is provided for solving the problem of online, portable and full-automatic type electromechanical engineering and biology compounding in the microorganism detection method.