The invention provides a latex enhanced immunoturbidimetry kit capable of inhibiting the rheumatoid factor interference. The kit comprises a reagent A and a reagent B. The reagent A is a solution capable of promoting the reactions between antigens and antibodies. The reagent B is a latex particle dispersion liquid containing sensitizing antibodies. The reagent A contains N-hydroxysuccinimide (1 to 40 g/L). The provided kit can effectively eliminate the interference of rheumatoid factor in a latex enhanced immunoturbidimetry sample on the immune-detection results; the accuracy and precision of measurement are both improved, and the kit has very good versatility.