The invention relates to a PCR fluorescent molecular beacon probe for detecting drug-induced deafness. A molecular beacon probe which is completely matched with a wild type sequence is designed for each locus; the condition of change in fluorescence intensity of each channel following the rising of the temperature is detected through high resolution melting curve analysis (HRM), so that Tm values of the melting of different genotypes of target fragments and the probe can be acquired; the wild type and mutant type have different Tm values, so that the purpose of typing a sample can be achieved; the system can accurately distinguish the genotypes on two loci, so that the purpose of simultaneously typing the two loci in a reaction can be achieved; the system is suitable for simultaneous detection for C1494T and A1555G mutations in mitochondria DNA and has the characteristics of accurate and reliable result, simple and convenient operation and low detection cost.