The invention discloses a PCN (Porous Coordination Network) 222 metal organic framework material as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing porphyrin, zirconium chloride and benzoic acid with N,N-dimethylformamide under the ultrasonic condition to obtain a mixed solution; carrying out hydrothermal reaction on the mixed solution, and carrying out ethanol washing, centrifuging and drying to obtain the PCN 222 metal organic framework material. The microtopography of the material is of a rod-like structure, and a crystal spindle is a hexahedron; the PCN 222 metal organic framework material has good thermal stability and can be used for enhancing the sensitiveness of a pesticide residue detection enzymatic inhibition method; the method disclosed by the invention has the advantages of simplicity, quickness and low cost and provides a practical tool for determining organophosphorus pesticide in a complex sample matrix.