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发明公开
CN106959290A 一种比率型稀土荧光探针及检测炭疽杆菌生物标志物的应用 无效 - 撤回

一种比率型稀土荧光探针及检测炭疽杆菌生物标志物的应用
摘要：
本发明公开了一种比率型稀土荧光探针Tb/DPA@SiO2‑Eu/GMP及可视化试纸制备方法以及其在检测炭疽杆菌生物标志物—2,6吡啶二羧酸（DPA）方面的应用。通过反向微乳液法合成该荧光探针后，利用时间分辨荧光分析方法实现对DPA浓度的定量检测。进一步，通过将滤纸浸泡在荧光探针溶液中，制备了一种便携式的可视化试纸，该试纸可应用于一种识别颜色的手机软件来检测DPA的浓度。本发明改变了目前常用的传统检测模式，无需复杂的操作过程，节约成本。本发明具有准确、灵敏、高度选择性的特点，制备的试纸具有可便携，方便使用，价格低廉等众多优点，具有广泛的应用前景。
摘要（英）：

The invention discloses a rate-type rare-earth fluorescent probe Tb/DPA@SiO2-Eu/GMP, a preparation method of visual test paper and an application thereof in the aspect of detecting bacillus-anthracis biomarker, namely 2, 6 dipicolinic acid (DPA). After the fluorescent probe is synthesized by a reverse microemulsion method, the quantitative detection for the concentration of the DPA is realized by utilizing a time-resolved fluorescence analysis method. Further, the portable visual test paper is prepared by soaking filter paper in a fluorescent probe solution, and can be applied to mobile-phone software for recognizing colors to detect the concentration of the DPA. The invention has the advantages that the currently-common traditional detection mode is changed, the complex operation process is not needed and the cost is saved. The invention has the characteristics of high accuracy, sensitivity and selectivity. The prepared test paper has the advantages of being convenient in carry and use, low in price and the like and has wide application prospect.

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