The invention discloses a traditional Chinese medicinal composition for preventing and treating chicken infectious bronchitis, and a preparation method and application thereof. The traditional Chinese medicinal composition is prepared from the following raw material medicines in parts by weight: 8 to 24 parts of elsholtzia penduliflora, 9 to 27 parts of championella sarcorrhiza, 15 to 45 parts of lepidogrammitis drymoglossoides ching, 3 to 9 parts of trollius macropetalus and 6 to 18 parts of adenia cardiophylla. The composition is prepared by the following steps: distilling the elsholtzia penduliflora and the trollius macropetalus, and collecting distillate; adding the championella sarcorrhiza, the lepidogrammitis drymoglossoides ching and the adenia cardiophylla into the residues and residual liquid after distillation, decocting for 2 to 3 times, combining the decoction, concentrating to be pasty, adjusting the ethanol until the alcohol concentration is 60 to 70 percent, precipitating, filtering, adding ethanol into the concentrated medicine liquid to adjust the alcohol concentration to be 75 to 80 percent, precipitating, filtering, concentrating filtrate, adding distilled water, performing cold storage, precipitating, filtering and concentrating; and combining the distillate the elsholtzia penduliflora and the trollius macropetalus with the extracting concentrated liquid. The traditional Chinese medicinal composition is used for preparing traditional Chinese veterinary medicines for preventing and treating chicken infectious bronchitis, has good effect and is safe and convenient.