The invention relates to a method for extracting and determining specific tobacco N-nitrosamine in cigarette main-stream smoke based on hydrophobic ion exchange solid-phase extraction and belongs to the technical field of chemical tobacco examination. The method for extracting the specific tobacco N-nitrosamine in the cigarette main-stream smoke comprises the steps that a filter element capturing total particulate matter of the main-stream smoke is put in an ammonium acetate solution, filtration is performed, and a HiCapt MCX solid-phase extraction column is adopted for solid-phase extraction to obtain the specific tobacco N-nitrosamine. By adopting the method for extracting the specific tobacco N-nitrosamine in the cigarette main-stream smoke, impurities and interferents can be effectively removed, the accuracy and precision of the determining method can be improved, the relative recovery rate of the method is 92.2%-108.9%, and the intra-day precision and the inter-day precision are smaller than 4.6% and 4.2% respectively.