The invention relates to an intelligent bus operation route arranging method which includes the following steps: receiving travel demands transmitted by users and position and operation states of intelligent buses; on the basis of the travel demands and the position and operation states of the intelligent buses within a region, determining an object intelligent bus; based on the travel demands and the position of the object intelligent bus, formulating an operation route; on the basis of the operation route and the travel demands, transmitting pick up stations of each user, the time when the intelligent buses are expected to arrive at the stations and plate numbers of the intelligent buses to respective user terminal; controlling the object intelligent bus to operate according to the operation route. The method herein can real-time formulate specific operation routes on the basis of users' travel demands, so that as long as a user provides travel demands, and the method can alert passengers of how to take the bus, and how to make transfer by scheduling and optimization of the system. Therefore, the method can achieve high quality service by providing minimum travel time and transfer and high system operation efficiency. The invention also provides an intelligent bus operation route arranging system.