The invention belongs to the field of preparation of catalysts and in particular relates to an ordered mesopore structure manganese cerium titanium catalyst as well as a preparation method and application thereof. The preparation method comprises the following steps: adopting a solvent evaporation induced self-assembly synthesis method, taking a triblock copolymer as a template agent, introducing a Mn/Ce active material assembly into a porous wall skeleton of a TiO2 carrier, thus obtaining the manganese cerium titanium catalyst with the ordered mesoporous structure. The active ingredients of the catalyst are high in dispersity, high in pore permeability, stable, ordered and large in specific surface area. The catalyst can be used for low-temperature denitration, multiphase catalytic degradation of organic matters and the like.