A method of using environment-friendly enzyme in the process of flue-cured tobacco planting includes: (1), after transplanting flue-cured tobacco, irrigating 1000-1500 times of environment-friendly enzyme liquid every 10-15 days, irrigating 800-100ml for each tobacco plant until the tobacco plants cap, and irrigating for 5-6 times in total; (2), after the tobacco plants enter a mature period, using 1000-1500 times of the environment-friendly enzyme liquid to spray leaves when the leaves on the upper portion, the middle portion and the lower portion are mature, wherein 50-60kg is used per mu. The method has the advantages that the method has obvious promoting effect on the tobacco plants and certain effect on controlling damage caused by tobacco brown spot, potassium content of tobacco leaves is increased remarkably, the method has obvious effect on improving quality of the tobacco leaves, first-class tobacco proportion based on the potassium content of the tobacco leaves is increased by 11.76%-0.02%, and output value per mu is increased by 628.96-159.17RMB.