The invention discloses a method for recycling oxytetracycline hydrochloride from oxytetracycline hydrochloride crystallization mother liquid. The method comprises the following steps: (1) adjusting the pH value of oxytetracycline hydrochloride mother liquid, and filtering to remove insoluble impurities; (2) separating treated filtrate by virtue of an ultrafiltration system so as to obtain a concentrated solution rich in oxytetracycline hydrochloride and an alcohol solution containing a small amount of calcium salt, and recycling methanol from the alcohol solution; (3) carrying out alkalization crystallization on the concentrated solution obtained through ultrafiltration, adding an alkalization reagent during alkalization so as to adjust the pH value, and recycling oxytetracycline, wherein the recycling rate of oxytetracycline reaches 93%-96%; and (4) adding recycled oxytetracycline into absolute methanol which is 5 times of volume dose of oxytetracycline, adding a small amount of calcium chloride to aid dissolving, filtering, adding hydrochloric acid methanol, and crystallizing, so as to obtain oxytetracycline hydrochloride. According to the method, inorganic salt, namely calcium salt, is filtered and separated by virtue of a membrane, the concentrated solution is subjected to alkalization crystallization to generate oxytetracycline, oxytetracycline is refined by virtue of hydrochloric acid ethanol so as to generate oxytetracycline hydrochloride, and the content of obtained oxytetracycline hydrochloride reaches 96%.