The invention discloses a measuring method for disturbance acceleration in attitude estimation of an aircraft. The method comprises the following steps: acquiring the attitude quaternion of the aircraft and acquiring measured acceleration in a body-axis coordinate system; acquiring disturbance acceleration in a reference coordinate system according to the attitude quaternion and the measured acceleration in the body-axis coordinate system and then acquiring discrete disturbance acceleration; and subjecting the discrete disturbance acceleration to segmented processing, then carrying out first-order low-pass filtering, and then transferring the processed discrete disturbance acceleration to the body-axis coordinate system for subtraction by the measured acceleration in the body-axis coordinate system so as to obtain accurate aircraft acceleration without the disturbance acceleration. Compared with the prior art, the measuring method provided by the invention is low in cost, needs no GPS sensor for measurement and can improve the attitude measurement precision of subsequent multi-sensor fusion technology.