The invention relates to the technical field of inter-network data transmission, and especially relates to a data duplex inter-transmission automation device between isolation networks based on a mobile storage medium. The device comprises a first network and a second network in boundary isolation, the first network and the second network are connected with a vein board robot through a mobile disk data line; the vein board robot is connected with the first network through an automation equipment control line; the vein board robot is connected with the mobile disk through the mobile disk data line. By use of above mentioned structure, the device disclosed by the invention has the advantage of automatically performing a security control operation of an encrypted label disk in comparison with the prior art; transmission requirements on the big data interaction by different projects can be satisfied.