The invention discloses a method for succulent plant propagation through a bacterium and application of the bacterium. The bacterium is actinomycetes of Homoserinibacter gongjuensis. The propagation method comprises the following steps: (1) explant sterilization and induction: taking explant, inoculating the explant in induction medium after sterilization and inducing to generate embryogenic callus; (2) inoculation and germination: jointly inoculating the Homoserinibacter gongjuensis bacterium and the embryogenic callus in germination medium to form normal cluster bud; (3) strong seedling and transplantation: inoculating the normal cluster buds cultured in the step (2) in strong seedling medium, culturing the cluster buds into strong cluster seedlings which can be transplanted and transplanting. The method disclosed by the invention breaks sterile environment required by general plant tissue culture and effectively solves the problems of general vitrification, slow growth speed and the like in a succulent plant tissue culture process by inoculating the symbiotic bacterium of Homoserinibacter gongjuensis in the succulent plant tissue culture process.