The invention relates to a preparation method and applications of an activity protector for coccidian oocysts in chicken dung. A composition is prepared from the following components in parts by weight: 1-10 parts of fructose, 1-10 parts of 10% polyvinyl pyrrolidone, 0.01-0.1 part of sodium azide, 0.01-0.05 part of 98% sulfuric acid, and 50-98 parts of sterile water. For the composition, the prescription is scientific and reasonable, the process is simple, the use is convenient, the cost is extremely low, the protection effect for coccidian oocysts in the faeces is good, with the ingredients including fructose as the protector for the chicken dung in the production of chicken coccidian vaccines, the activity of coccidian oocysts in the chicken dung can be effectively kept, the storage time of the faeces can be prolonged, the large-scale production is facilitated, the production efficiency can be improved, and after the faeces are stored for 4 days by adopting the protector, no differences exist between the activity of the oocysts and the activity of the oocysts in the fresh faeces.