The invention discloses a detection method for staphylococcus aureus. The detection method comprises the following steps: preparing a double-fluorescence-label probe with two carboxy flouorescein labels from a first probe, a second probe and a single-chain DNA; mixing the double-fluorescence-label probe with a graphene oxide solution, thus forming a compound solution; extracting the total RNA of a bacteria solution to be detected; simultaneously adding the total RNA and deoxyribonuclease into the compound solution, thus forming a mixed solution, and incubating the mixed solution; measuring a fluorescence value of the incubated mixed solution, and calculating the concentration of the staphylococcus aureus according to the fluorescence value. According to the detection method disclosed by the embodiment of the invention, a section of specifically recognized staphylococcus aureus 16S rRNA is used as a target to design the probe, and a double-fluorescent-label probe/graphene oxide system is built based on the fluorescence superposition principle; cyclic detection on the target is realized according to the characteristic of the deoxyribonuclease, so that a fluorescence signal is amplified, the sensitivity of the detection method for the staphylococcus aureus is improved, and the detection efficiency of the detection method is also improved.