The invention discloses a tissue culture method of fruits of lonicera fragrantissima subsp. Standishii. Totipotency of vegetable cells is utilized, plants which have good growth momentum and bear big fruits are adopted as explant, through the steps of induction culture, subculture, rooting culture, acclimatization and transplant and the like, the seedlings of the fruits of lonicera fragrantissima subsp. Standishii are obtained after culturing, the seedlings of the fruits of lonicera fragrantissima subsp. Standishii which have excellent genes can be cultured in large scale, the conditions like temperatures, illumination, nutrients in all culturing phases are controlled, and thus a large number of the plants of the fruits of lonicera fragrantissima subsp. Standishii. Totipotency of vegetable cells can be cultured within a short time.