The invention discloses a comprehensive matched new method for penaeus vannamei bred in fresh water. The method comprises the step that in the breeding pond of the penaeus vannamei, gurnards, catfishes and margarya melanioides are subjected to matching stocking, wherein the stocking size of the gurnards is 100 g-150 g per fish, the stocking rate is 10-15 per mu, the stocking size of the catfishes is 100 g-200 g per fish, the stocking rate is 20-30 per mu, and the stocking rate of the margarya melanioides is 50-100 kg per mu. Accordingly, the death rate of penaeus orientalis is effectively decreased, growth of the fishes is promoted while the penaeus orientalis bait utilization rate is increased, and the mouthfeel of fish meat is optimized.